ISOLATION, IDENTIFICATION AND ANTIMICROBIAL ANALYSIS OF PSEUDOMONAS AERUGINOSA FROM DIFFERENT CLINICAL SAMPLES AND TO STUDY THE INTERACTIONS OF TARGET AND RESISTANT PROTEINS WITH CEFEPIME AND IMIPENEM THROUGH DOCKING
Objective: The present study aims towards gender wise distribution of P. aeruginosa and detection of antimicrobial
analysis against various isolates from different clinical samples and to better understand the interaction of antibiotics
with the resistant proteins and sensitive proteins of this pathogen using Docking.
Study Design: Analytical/Experimental study.
Place and Duration of Study: The research study was carried out at Microbiology laboratory of Shaheed Benazir
Bhutto Women University, Peshawar from July to October 2014.
Methodology: A total of 79 different clinical samples comprising of fourty-five early morning mid stream urine samples,
twenty-eight pus samples, five blood and one stool specimens were processed. MacConkey agar and CLED (Cysteine
Lactose Electrolyte Deficient) agar were used as growth media for the culturing of microorganisms. Microorganisms
were then identified through Gram staining followed by microscopy. Biochemical tests such as Urease, TSI (triple
sugar iron), simmon citrate and Oxidase were carried out. Antibiogram analysis was accomplished using Kirby- Bauer
antibiotic disk diffusion method.
Results: The prevalence rate of P. aeruginosa in the present study was found to be 5.06% where female patients
constituted a larger group with 75% as compared to male (25%). Antibiotic susceptibility test results showed that all
of the P. aeruginosa isolates were fully resistant (100%) to augmentin, imipenem, and erythromycin. They exhibited
moderate resistant to cefotaxime and were fully sensitive (100%) to piperacillin-tazobactam, cefepime, cefobid, amikacin,
gentamycin, and norfloxacin, while 75% sensitivity was shown to ciprofloxacin and 50% sensitivity was shown
against ceftriaxone, aztreonam and moxifloxacin. GLN547, GLU568, GLU550 and THR565 of penicillin binding protein
of P. aeruginosa are involved in providing sensitivity to cefepime. Whereas TRP87, ASP119, GLU225 and ASN233 of
Metallo β-Lactamase of P. aeruginosa are involved in developing resistance to imipenem.
Conclusion: It was concluded that P. aeruginosa was present in high percentage in female ant it showed resistance
towards many first generation drugs. Developing resistance towards third generation drugs was also noticed. Docking
showed strong interactions among selected drugs and respective proteins.
Keywords: Antibiotic susceptibility, Pseudomonas aeruginosa, Penicillin Binding Protein, Metallo β-Lactamase.