ANALYSIS OF CLINICAL SAMPLES FOR ESCHERICHIA COLI ALONG WITH MOLECULAR DOCKING OF PBP1B WITH AMIKACIN AND CTX-M 14 WITH CEFTAZIDIME

ABSTRACT
The present study was undertaken to determine the prevalence of E. coli and its antibiogram analysis using disc
diffusion method and to study the interactions between target and resistant proteins with drugs through docking.
A total of 80 samples were obtained from different Hospitals of Peshawar and were processed in the Laboratory of
Microbiology Department, Shaheed Benazir Bhutto Women university Peshawar, from July to September, 2014 for E.
coli identification. All samples were streaked on MacConkey and Blood agar while urine was streaked on CLED agar.
E. coli identification was based on cultural characteristics, gram staining and biochemical tests. Analysis of clinical
samples revealed no growth on Sputum and blood sample while puss had different bacterial growth in which account
of E. coli was much lower i.e. 12% while higher percentage of this isolate was found in urine 21.4%. E. coli occur more
frequently with enhanced percentage in females than males. Antibiotic sensitivity was performed using 14 antibiotics
by disc diffusion method in which E. coli show maximum susceptibility towards imipenem (91.6%), Amikacin (83.3%)
and meropenem (83.3%). While maximum resistance was seen towards erythromycin (100%), Ceftazidime (83.3%)
and cefotaxime (75%). To measure the distances, docking of Amikacin and Ceftazidime with PBP 1b and CTX-M 14
enzyme were performed. Minimum distance of docked complex of Amikacin-PBP1b showed active action of drug that
target the major component of cell wall synthesis while smallest distance between Ceftazidime and CTX-M 14 showed
the attack of this enzyme to drug for the purpose of inactivation. This finding is useful for the selection of effective drug
for empirical treatment and for prevention and control of the infection.
Keyword: Urinalysis, UTI, Uropathogenic Escherichia coli, Antibiogram analysis, PBP1b, CTX-M 14, Molecular docking