COMPARISION OF COLORIMETRIC AND HPLC METHODS FOR EVALUATION OF HBA1C IN DIABETIC PATIENTS

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Abstract

ABSTRACT
Objectives: To measure the HbA1c by colorimetric and HPLC methods and to evaluate the analytic sensitivity of both
the methods in diabetic patients.
Material and Methods: It was a cross sectional analytical study conducted in Khyber Teaching Hospital and Khyber
Medical College Peshawar from September 2014 to November 2014. A total of 90 individuals were included in the study
and divided in two groups, one consisting of 60 known diabetics already on treatment and one consisting of 30 non
diabetic individuals. The HbA1c level of both the groups was measured by both the colorimetric and HPLC methods.
Results: Mean + SD by colorimetric method in non diabetic individuals was 5.79 + 0.55 percent while that by HPLC
method was 5.3 + 0.80 percent. On other hand
in known diabetic patients category (Group 2), it was seen that the Mean + SD by colorimetric method was 7.11 +
1.54 percent while that by HPLC was 8.68 + 2.93 percent. Correlation is highly significant at the 0.01 level (2-tailed)
Conclusion: This study indicated that HbA1c should be detected by HPLC method in order to get the actual picture
of glycemic control of the patient and to manage the patient accordingly.
Key words: HbA1c, Colorimetric method, High-performance liquid chromatography.

 

Authors: 
Ahmed Rafique
Arshad Parvez2
Mohsin Shafi1
Munir Hussain1
Khalid Mehmood2

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