QUINOLONES RESISTANCE AMONG ESCHERICHIA COLI ISOLATES FROM CLINICAL SPECIMENS

Objective: This study was aimed to isolates the Quinolones resistance Escherichia coli from clinical samples
including urine, pus and HVS at Hayatabad Medical complex over a period of 4 months.
Material and Methods: A total of 240 samples were processed for isolation of E. coli in which 48 (20%) were positive
for E. coli isolates. Seven Quinolones antimicrobial drugs namely Nalidixic Acid, Ciprofloxacin, Levofloxacin,
Norfloxacin, Ofloxacin, Pefloxacin, and Moxifloxacin were used during the study. Antimicrobial activity was tested by
standard Disk Diffusion (Kirby-Bauer) method.
Results: The prevalence of E. coli in urine sample was 37.5%, Pus 12.5% and HVS 10%. The gender wise distribu-
tion of E .coli was higher in females than males. Resistant pattern of E. coli shows that it is highly resistant to Nalidixic
acid 95.8% and least resistance shown to Ciprofloxacin 54%. Ciprofloxacin is a drug of choice which shows 39.5%
sensitivity to all isolates of E. coli, whereas, Nalidixic acid showed high resistance of 95.5%.
Conclusion: This study concluded that E. coli is a dominant microorganism among the clinical specimens in HMC.
The E. coli is present in females more than males. More than 60% resistance is shown by all Quinolones toward
E.coli. Ciprofloxacin is a drug of choice and shows 39.5% sensitivity to all isolates of E. coli, whereas, Nalidixic acid
showed high resistance 95.5%.
Key words: Quinolones, E. coli, Antibiotics, Resistance.