COMPARATIVE STUDY OF BACTERIAL PATHOGENS CAUSING URINARY TRACT INFECTIONS IN DIABETIC AND NON DIABEIC PATIENTS

Objective: To identify and compare different bacterial pathogens causing UTI (urinary tract infections) in diabetic
and non-diabetic patients.
Study Design: Prevalence / Comparative study.
Place and Duration of Study: Microbiology Laboratory, Department of Microbiology, Abasyn University Peshawar,
from July 2010 to November 2010.
Methodology: A total of 60 samples were processed, 30 of them for diabetic and the rest for normal patients suffering
from UTI. Five to ten ml fresh midstream urine was collected aseptically in a sterile urine container. Blood agar,
MacConkey agar and CLED (Cysteine Lactose Electrolyte Deficient) agar were used as growth media for the culturing
of microorganisms. Microorganisms were then identified through Gram staining followed by microscopy, catalase,
coagulase, urease, TSI (triple sugar iron), Indole and Oxidase tests.
Results: Out of 60 cases, 38 (63.33%) cases were found positive in which18 (47.37%) were from non-diabetic
patients and 20 (52.63%) were from diabetic patients. Eleven samples (61.11%) were found positive for females out
of 18 positive samples of non-diabetic patients, while seven samples (38.89%) were found positive for males.
Similarly, in case of 20 positive samples of diabetic patients, eighteen samples (90%) were from females while two
samples (10%) were from males. On the other hand, in non-diabetic patients, E.coli were isolated from 50% samples,
Citrobacter and Staphylococcus aureus from 16.6% each, Klebseilla from 11.1% and Providencia species from 5.5%.
Accordingly, in case of diabetic patients, Proteus species were isolated from 66.66% samples, Pseudomonas from
20%, Klebseilla from 15% and Morganella from 5%. Generally, female population was found more infected (76.2%)
with urinary tract infections than males (23.8%).
Conclusion: The study concluded that a high rate of UTI is prevalent in our community not only in healthy population
but also in diabetic patients.
Keywords: UTI. Diabetic and Non-diabetic Patients. Gram-Positive and Gram-Negative Bacteria.